Open AccessA novel PRE-element from Drosophila virilis genome as a useful model silencer
Author(s) -
Darya Chetverina,
А. В. Михайлова,
P. G. Georgiev,
M. M. Erokhin
Publication year - 2019
Publication title -
doklady akademii nauk. rossijskaâ akademiâ nauk
Language(s) - English
Resource type - Journals
ISSN - 0869-5652
DOI - 10.31857/s0869-56524843363-366
Subject(s) - drosophila melanogaster , p element , drosophila virilis , genome , biology , transposable element , genetics , gene , drosophila (subgenus) , melanogaster , drosophilidae , transposition (logic) , linguistics , philosophy
We tested the activity of the PRE element from the Drosophila virilis genome, which is the homologue of the known Drosophila melanogaster bxdPRE element from the regulatory region of the Ubx gene. It is easy to select unique primers to this element that do not occur in the Drosophila melanogaster genome. We showed that the studied PRE element causes a strong repression of the white marker gene upon insertion into the Drosophila melanogaster genome and interacts with the PcG proteins of the PRC1 and PhoRC complexes.