Open AccessExpression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli
Author(s) -
Le Thi Tuyet Cham,
Vu Ngoc Thang,
Trần Anh Tuấn,
Vu Thi Thuy Hang
Publication year - 2020
Publication title -
agricultural sciences
Language(s) - English
Resource type - Journals
ISSN - 2588-1299
DOI - 10.31817/vjas.2020.3.1.03
Subject(s) - arabidopsis thaliana , zinc finger , escherichia coli , biology , arabidopsis , gene , gene expression , expression vector , regulator , abiotic stress , microbiology and biotechnology , genetics , mutant , recombinant dna , transcription factor
The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.