Open AccessPHYTOCHEMICAL SCREENING, CYTOTOXIC ACTIVITY AND MOLECULAR DOCKING STUDIES OF Eclipta alba LEAVES EXTRACT AGAINST ORAL CANCER
Author(s) -
Lavanya Jayaraman,
Subhashini Shivaji,
Shanmugam Anandakumar
Publication year - 2022
Publication title -
rasayan journal of chemistry/rasayan journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.281
H-Index - 22
eISSN - 0976-0083
pISSN - 0974-1496
DOI - 10.31788/rjc.2022.1516754
Subject(s) - phytochemical , cytotoxicity , traditional medicine , terpenoid , docking (animal) , chemistry , mtt assay , in silico , pharmacology , biochemistry , biology , in vitro , medicine , nursing , gene
The goal of this research is to identify phytochemical ingredients in the methanol extract of Eclipta alba (E. alba) leaves and assess their cytotoxicity as well as in-silico binding mechanism of action against oral squamous cell carcinoma (OSCC). The GC-MS analysis has confirmed the presence of major phytochemicals like steroids, terpenoids, tannins etc., in the leaf extract of E. alba. The cytotoxicity activity of the extract by MTT assay showed dose-dependent inhibition (96% at 300 µg/ml) towards the oral cancer KB cell line. Molecular docking of important molecules such as palmitic acid, 9, 12-Octadecadienoic-acid, and Stigmasta-3, 5-dien-7-one with target proteins including Akt1 and Akt2 exposed the best binding energy. The leaves extract of E. alba will be an herbal remedy for oral cancer containing potential inhibitors owing to proven its cytotoxicity activity as well has revealed its binding efficacy against target proteins.