Open AccessGLYCOSYLATED FLAVONOID COMPOUND FROM COCOA PARASITE LEAVES (Dendrophthoe pentandra (L.) Mig.)
Author(s) -
H. Br. Sembiring,
L. Marpaung,
M. Basyuni,
P. Simanjuntak
Publication year - 2022
Publication title -
rasayan journal of chemistry/rasayan journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.281
H-Index - 22
eISSN - 0976-0083
pISSN - 0974-1496
DOI - 10.31788/rjc.2022.1516382
Subject(s) - ethyl acetate , flavonoid , chemistry , quercetin , quercitrin , chromatography , dept , phytochemical , column chromatography , organic chemistry , traditional medicine , stereochemistry , antioxidant , biochemistry , medicine
Dendrophthoe pentandra (L.) Mig) is a parasitic plant that grows on cacao trees (Theobroma cacao L). Methanol extract of D. pentandra (L.) Mig) leaves had an IC50 value of 30.31 µg/mL as antioxidant activity and the results of phytochemical screening were contained flavonoid compounds. The aim of this research is to isolate and identification of a glycosylated flavonoid compound from D. pentandra (L.) Mig) leaves. Extraction of D. pentandra (L.) Mig) leaves powder using methanol was performed by maceration. The crude extract was partitioned with ethyl acetate and the flavonoid compound in the ethyl acetate extract was separated by column chromatography [(SiO2, i). CHCl3; ii). CHCl3-MeOH = 10: 1 ~ 1: 1 iii). Preparative TLC (CHCl3-ethyl acetate = 1: 4)] yielded a compound A. Based on the interpretation of ultraviolet (UV), infrared (FT-IR), Nuclear Magnetic Resonances spectra [NMR 1 D (1H- & 13C-, DEPT), 2 D (COSY and HMBC)], compound A was determined as quercitrin (quercetin 3-O-ramnosida).