IMPACT OF AMMONIUM PYROLIDINE DITHIOCARBAMATE ON BIOMECHANICAL CHARACTERISTICS OF FEMORAL BONE IN RATS UNDER THE CONDITIONS OF COMBINED EXCESSIVE INTAKE OF FLUORIDE AND SODIUM NITRATE

This article describes the effect of ammonium pyrrolidine dithiocarbamate, an inhibitor of NF-κB activation, on the biomechanical characteristics of femoral bones in rats under the conditions of combined excess intake of fluoride and sodium nitrate. The series of experiments were performed on 30 white Wistar rats weighing 190-240 g, divided into 3 groups: 1st group included intact animals, 2nd group included animals exposed to the combined administration of sodium fluoride (10 mg/kg body weight) and sodium nitrate (500 mg / kg body weight) for 30 days; the 3rd group included rats, who starting from the 15th day of intoxication were injected with an inhibitor of NF-κB activation, ammonium pyrrolidine dithiocarbamate is a dose of 76 mg / kg 3 times a week. The investigation of the biomechanical properties of the femur was performed according to a 2-point scheme (linear rupture test) and a 3-point scheme (bending test) using a rupture machine RMU-0.05-1 with an assessment of rupture load (strength) and bone elongation (elasticity); we also applied a 4-point scheme (bending test) using the deformation test setup MRC-1 with following calculation of the Young's modulus, tensile strength, relative residual deformation to failure and relative elongation to failure. The administration of ammonium pyrrolidine dithiocarbamate under the conditions of the experiment significantly increased the tensile load at linear rupture and bending by 35.3 and 23.5%, respectively, compared with the results in the 2nd group. The relative elongation of the femoral bones in the test for linear rupture and flexion was more pronounced than the values ​​of the 2nd group by 7.9 and 18.6%. When testing for bending according to the 4-point scheme using the deformation setup MRK-1, we revealed a probable increase in the Young's modulus of elasticity by 19.8% compared to the results in the 2nd group. However, the indicators of the limit of elasticity and strength, the relative elongation of the extreme fibers before fracture did not change significantly. This enables us to conclude that ammonium pyrrolidine dithiocarbamate, the inhibitor of the NF-κB transcription factor, used under the conditions of combined administration of fluoride and sodium nitrate can serve an effective osteoprotective agent, which increases the strength and elasticity of the femoral bones.