Open AccessSENSITIVITY OF STREPTOCOCCUS PLURANIMALIUM CLINICAL ISOLATE TO ANTISEPTICS
Author(s) -
M. O. Faustova
Publication year - 2020
Publication title -
aktualʹnì problemi sučasnoï medicini: vìsnik ukraïnsʹkoï medičnoï stomatologìčnoï akademì
Language(s) - English
Resource type - Journals
eISSN - 2077-1126
pISSN - 2077-1096
DOI - 10.31718/2077-1096.20.2.182
Subject(s) - chlorhexidine , serial dilution , antimicrobial , microbiology and biotechnology , antibiotic sensitivity , minimum inhibitory concentration , streptococcus , microorganism , medicine , antibiotics , food science , bacteria , biology , dentistry , genetics , alternative medicine , pathology
Recent reports have stressed out a global increase in the identification of new pathogens, which are not characteristic for the human microbiota, as well as significant changes in the microbial composition of different biotopes of its body. The purpose of this study was to investigate the sensitivity of S. pluranimalium clinical isolate to antiseptics. Materials and methods. The clinical isolate of S. pluranimalium was taken from the patient's mouth. The isolation and cultivation of the microorganism was carried out by standard techniques. Final identification was performed using an automatic bacteriological analyzer. The sensitivity of the studied S. pluranimalium isolate to antiseptics was determined by the method of double serial dilutions in liquid nutrient medium, in accordance with the Order №167 of the Ministry of Public Health of Ukraine on “On Approval of Training Guidance” Assessment of the sensitivity of microorganisms to antibiotics”. The sensitivity was assessed by the minimum inhibitory and minimum bactericidal concentrations in µg / ml. The sensitivity of the clinical isolate under the study was determined to 0.05% for chlorhexidine, 0.05% for decasan, and 2.0% for chlorophyllipt extract. According to the results obtained, the clinical isolate of S. pluranimalium was sensitive to the action of antiseptics in varying degrees. The antiseptics of the Quaternary ammonium compound group had a more pronounced antimicrobial effect on the test organism compared to the chlorophyllipt extract. Conclusions. Among these antiseptics, the tested strain of streptococcus was the most sensitive to decasan, compared with chlorhexidine and natural extract of chlorophyllipt, the minimum inhibitory and minimum bactericidal concentrations of which exceeded these indicators of decasan.