Open AccessDYNAMICS OF HEMATOLOGICAL INDICES IN RATS AFTER ADMINISTRATION OF DIAMOND-CARBON COATED STEEL IMPLANTS INTO THE FEMUR
Author(s) -
Vasyl Makarov,
Д. В. Морозенко,
K. B. Gliebova
Publication year - 2019
Publication title -
aktualʹnì problemi sučasnoï medicini: vìsnik ukraïnsʹkoï medičnoï stomatologìčnoï akademì
Language(s) - English
Resource type - Journals
eISSN - 2077-1126
pISSN - 2077-1096
DOI - 10.31718/2077-1096.19.3.138
Subject(s) - implant , medicine , white blood cell , femur , surgery
. The pathological body response to metal implants is considered to be well known. There are a lot of studies devoted to this issue. This work is devoted to investigating the dynamic changes of haematological indicators as markers reflecting body response to the insertion of steel implants with diamond-carbon coating in rats. The purpose of the study is to assess the effects caused by steel implant with a diamond-coated coating on rat’s body by assessing hematological parameters. Materials and methods. The study involved 61 male rats: five of which were intact animals, and the rest was divided into two groups (28 animals in each). The first group was the control group, in which rats were inserted non-coated steel implants into the femoral metaphysic; the second group was test group, in which rats were inserted steel diamond-coated implants). The age of animals at the beginning of the experiment was 5–6 months, the body weight was 300–400 g. Blood for research was taken from the animals after decapitation in 7, 14, 30 and 90 days following the implantation. The blood samples were measured for the red blood cell, white blood cells, haemoglobin, leukogram. The findings obtained were processed by statistical analysis methods. Results and conclusions. The first group of the rats with inserted steel implants without coating showed the number of erythrocytes and haemoglobin did not differ from those in the intact rats, the leukocyte count on the 7th and 14th days was reduced by 31.8 % to 39.4 %, respectively; on the 7th day the percentage of lymphocytes and monocytes increased by 13.0 % and 5.0 % due to the toxic immunosuppressive effect of steel implants on the body of the experimental animals. The 2nd group of rats, which were inserted diamond-coated steel implants in their femurs, the rates of erythrocytopoiesis, the leukocyte number and leukogram characteristics remained unchanged during all the observation periods, except for an increase in the percentage of eosinophils on the 7th day and lymphocytes on the 90th day of follow- by 3 % that indicates a less pronounced body response in comparison with the animals of I group.