QUANTITATIVE CHARACTERISTICS OF GENERAL MICROBIAL COLONIZATION OF ORAL MUCOSA DURING ADAPTATION TO ACRYLIC REMOVABLE PARTIAL DENTURE

Relieving the process of dentures adaptation and, as a result, the quality of prosthetic care arouses growing interest to the investigation of the adhesion and microbial contamination of the bases of removable dentures made of various materials. The purpose of this work was to study the oral microbial colonization during adaptation to removable partial dentures with an acrylic basis. Materials and methods. The study involved 15 patients. The first group consisted of 10 patients with dentition defects who were recommended to wear removable partial dentures for dentition correction. The comparison group included 5 patients with sanitized oral cavity, who did not need any orthodontic treatment. To study aerobes and facultative anaerobes of oral mucosa, sampling of material for microscopy was performed using the sterile dental paper pin (No. 30) from a total area of 1 cm2 with following of microorganism culturing in a nutrient medium at 37 °C. The microbial population of the oral mucous was determined on Petri plates by counting the number of colony-forming units per 1 cm3 of biomaterial taken from patients before denture wearing, and then in 3 and in 4 days of denture wearing. The results obtained were statistically processed by the standard software package Microsoft Excel 2016, the analysis of reliability was performed by Student’s t-test. Results. Wearing removable partial dentures with a basis of acrylic plastic contributed to a significant increase in the total microbial colonization of the mucous membranes of the oral cavity by the third day of denture wearing. Moreover, the number of colony-forming units/cm3 on the oral mucosa under the dentures steadily was growing during the next two weeks of denture adaptation.