Open AccessANTIGENICITY OF BRUCELLA PROTEINS BY THE ELISA TEST
Author(s) -
Aitbay Bulashev,
Alfiya Syzdykova,
Zh. A. Suranshiyev,
Кanat Tursunov,
Saule Eskendirova
Publication year - 2019
Publication title -
vestnik novosibirskogo gosudarstvennogo agrarnogo universiteta
Language(s) - English
Resource type - Journals
ISSN - 2072-6724
DOI - 10.31677/2072-6724-2019-50-1-92-100
Subject(s) - antigenicity , brucella , brucellosis , serology , brucellaceae , recombinant dna , antibody , antigen , biology , brucella melitensis , microbiology and biotechnology , virology , periplasmic space , bacterial outer membrane , immunology , escherichia coli , biochemistry , gene
Lifetime diagnostics of animal brucellosis is mainly based on serological reactions as SAT, RBPT and CFT. The tests determine antibodies by means of antigen produced from Brucella S-cells that mainly contain lipopolysaccharides (LPS). The LPS may cause cross-reactions with other clinically significant gram-negative bacteria; this leads to false-positive results. Due to this fact, the researchers involved in improving. The paper highlights the research results on antigenicity of 5 recombinant Brucella proteins (rOMP19, rOMP25, rOMP31, rBP26 and rSOD) and soluble protein preparations (CSP) of B. abortus and/or B. melitensis by indirect ELISA using cattle and sheep serum samples positive for brucellosis by classical serological tests. CSP appeared to be the most antigenic among the protein specimens; it determined antibodies in 94.8% of the cattle and 69% of sheep. Antibodies which were specific to rOMP19, rOMP25 and rOMP31 were detected in 39%; 50.6 and 76.6% of antibody-positive cows. Periplasmic proteins (rBP26 and rSOD) were observed as less antigenic than outer membrane proteins and revealed anti-Brucella antibodies in 29.9 and 14.3% of the cattle. Recombinant proteins were not detected by antibodies of sheep positive for brucellosis. Antibodies to recombinant proteins by i-ELISA were detected in the small number of the cattle kept at brucellosis free farm (from 2.1 to 12.5%). The results obtained outline the necessity to carry out experimental infection of animals in order to assess properly the capacities of recombinant proteins when diagnosing brucellosis.