Open AccessFermentative activity in the leaves of garden roses in conditions of humid subtropics of Russia
Author(s) -
K. V. Klemeshova,
A. A. Budarin,
Н. Н. Карпун
Publication year - 2019
Publication title -
sadovodstvo i vinogradarstvo
Language(s) - English
Resource type - Journals
eISSN - 2618-9003
pISSN - 0235-2591
DOI - 10.31676/0235-2591-2019-5-28-32
Subject(s) - catalase , peroxidase , horticulture , botany , subtropics , abiotic component , biology , botrytis cinerea , polyphenol oxidase , biotic component , enzyme assay , antioxidant , enzyme , ecology , biochemistry
The increased activity of enzymes that counteract the oxidative degradation of substances is one of the physiological and biochemical adaptations of plants to biotic and abiotic factors. The aim of the research was to study the peculiarities of the antioxidant system of garden roses to elaborate a methodology for a comprehensive evaluation of culture in the wet subtropics of the Russian Black Sea coast. The objects of study were garden rose varieties ( Rosa × hybrida hort.), belonging to functional groups – bush and large-flowered, and differing in the degree of resistance to the main fungal diseases of the culture in the region ( Diplocarpon rosae FA Wolf, Podosphaera pannosa (Wallr.) De Bary, Botrytis cinerea Pers., Phragmidium mucronatum (Pers.) Schltdl.) The activity of the antioxidant system was evaluated by the activity of the enzymes catalase and peroxidase (guaiacol). Catalase activity (CAT) in physiologically mature rose leaves was determined by the gasometric method according to I. I. Gunar, peroxidase (GPO) by the modified method according to I. P. Ermakov, according to the absorption spectrum (wavelength – 440 nm), recorded on the spectrophotometer PE-5400vi. The studies were carried out in dynamics during the growing season in 2016-2017. The article presents data on changes in the activity of enzymes of the primary antioxidant system – catalase and peroxidase, in the leaves of bush and large-flowered varieties of garden roses, which differ in the degree of resistance to the main phytopathogens of the culture in the region. Variety differences in the activity of enzymes, the dynamics of changes in the activity of antioxidant enzymes during the growing season, the dependence of the enzymatic activity on the hydrothermal conditions of the humid subtropical climate of the Sochi Black Sea Region have been determined. Diagnostic parameters have been established to develop the methodology for a comprehensive evaluation of landscape gardening roses in the region. When developing methods for a comprehensive evaluation of garden roses, the activity of guaiacol peroxidase can be used to analyze the adaptive potential of the culture in the region.