Open AccessTransport mechanism of eurycomanone from Eurycoma longifolia Jack across Caco-2 cells model
Author(s) -
Wu-Yan Guo,
Huan Zhang,
YueYang Zhang,
Shuyan Wang,
Jing Ren,
Yongqing Jia,
Rui Li,
Bo Zhang
Publication year - 2022
Publication title -
journal of food bioactives
Language(s) - English
Resource type - Journals
eISSN - 2637-8779
pISSN - 2637-8752
DOI - 10.31665/jfb.2022.17301
Subject(s) - paracellular transport , bioavailability , chemistry , caco 2 , passive transport , absorption (acoustics) , verapamil , pharmacology , biophysics , permeability (electromagnetism) , biochemistry , cell , calcium , membrane , medicine , biology , materials science , organic chemistry , composite material
Eurycomanone is the main active ingredient of Eurycoma longifolia Jack with anti-cancer, anti-malaria, improving male sexual dysfunction and other effects.The poor lipid solubility of eurycomanone is potential reason for its low bioavailability. However, the transmembrane absorption mechanism has not been reported. In this study, the Caco-2 cell monolayer model was used to investigate the influence of different factors on the eurycomanone transmembrane absorption, including time, concentration, temperature, P-glycoprotein inhibitors (verapamil or cyclosporin A) and collateral transport enhancer (sodium desoxycholate or EDTA).The results showed that the apparent permeability coefficient (Papp) of eurycomanone was lower than 10-6 cm/s with poor oral absorption. The Papp was not dependent on concentrations or temperatures. The addition of paracellular transport enhancer promoted the absorption of eurycomanone(P < 0.05), whereas p-glycoprotein inhibitors had no effect.Taken together, this study indicated that absorption of eurycomanone may undergo passive transmembrane transport and paracellular transport.