Open AccessIdentification of a compound, at a very low dose (less than 100 ng/g of diet), with lifespan-elongation activity towards SOD-1 mutant adults of Drosophila melanogaster in the hot water extract of Chlorella pyrenoidosa
Author(s) -
Yifeng Zheng,
Yoshihiro Inoué,
Nagi Kohno,
Masaki Fujishima,
Eri Okumura,
Kenji Satô
Publication year - 2020
Publication title -
journal of food bioactives
Language(s) - English
Resource type - Journals
eISSN - 2637-8779
pISSN - 2637-8752
DOI - 10.31665/jfb.2020.9218
Subject(s) - drosophila melanogaster , phenethylamine , chemistry , melanogaster , mutant , antioxidant , biochemistry , stereochemistry , gene
Hot water extract of chlorella (WEC) increased the lifespan of superoxide dismutase (SOD)-1 mutant adults of Drosophila melanogaster in a dose dependent manner (200–800 µg/mL). Compounds in WEC were successively fractionated by solid phase extraction using a Sep-Pak C18 cartridge and size exclusion chromatography (SEC). Amino compounds in SEC fractions were derivatized with
6-aminoquinolyl-N-hydroxylsuccinimidyl carbamate and analyzed by reversed phased-liquid chromatography-tandem mass spectrometry. Phenylalanine, phenethylamine, isopentylamine, and 2-methylbutylamine were identified in the SEC fraction, which increased the lifespan of the D. melanogaster mutant adults. Phenethylamine, at very low doses (6–60 ng/g of diet) that roughly corresponded to those of phenethylamine in WEC (200–800 μg/mL), increased the lifespan of the D. melanogaster adults, while isopentylamine did not exert the lifespan elongation activity. Since phenethylamine did not show SOD-like activity, it did not increase lifespan by direct antioxidant activity.