Open AccessEnhancement of Melanocortin-4 Receptor (MC4R) and Constancy of Kiss1 mRNAs Expression in the Hypothalamic Arcuate Nucleus in a Model of Polycystic Ovary Syndrome Rat
Author(s) -
Mohammad Hossein Nooranizadeh,
Farhad Rahmanifar,
Somayeh Ahmadloo,
Zahra Shaaban,
Mehdi Shirazi,
Amin Tamadon
Publication year - 2018
Publication title -
galen medical journal
Language(s) - English
Resource type - Journals
eISSN - 2588-2767
pISSN - 2322-2379
DOI - 10.31661/gmj.v7i0.1070
Subject(s) - polycystic ovary , endocrinology , medicine , kisspeptin , hypothalamus , corpus luteum , arcuate nucleus , melanocortin 4 receptor , arc (geometry) , hyperandrogenism , ovary , melanocortin , receptor , biology , insulin , insulin resistance , geometry , mathematics
Background: Hypothalamic Melanocortin-4 Receptor (MC4R) and kiss1/kisspeptin systems play roles in reproductive processes. This study was conducted to evaluate changes in MC4R and kiss1 genes expression in the arcuate nucleus (ARC) of the hypothalamus and its relationship with polycystic ovary syndrome (PCOS) in rats.Materials and Methods: In the current experimental study, 24 female rats were randomly and equally allocated into nulliparous and primiparous groups and then were divided into two subgroups of PCOS and control. PCOS was induced by exposure to continuous light. Sex-related hormones were evaluated by radioimmunoassay or immunoradiometric assay. Expressions of MC4R and kiss1 gene in the ARC of the hypothalamus of the rats were evaluated by real-time PCR. Histomorphometric alterations of ovaries were compared between groups.Results: Number of tertiary follicles and their size and number of atretic follicles in the PCOS subgroups were more than those in the controls (P 0.05).The kiss1 mRNA expression in the PCOS and control subgroups was not significantly different (P>0.05).Conclusion: Overexpression of MC4R gene after PCOS induction in the ARC of the hypothalamus may link to metabolic disorders of induced PCOS in the rats. However, alteration in the kiss1 mRNA expression after PCOS induction was not observed in the rats. [GMJ.2018;7:e1070]