ANALYSIS OF THE CORRELATION BETWEEN ADVANCED GLYCATION END-PRODUCTS LEVELS AND THE CYTOKINE PROFILE IN PATIENTS WITH ALLERGIC DISEASES ASSOCIATED WITH CHRONIC EPSTEIN-BARR VIRAL INFECTION

ANALYSIS OF THE CORRELATION BETWEEN ADVANCED GLYCATION END-PRODUCTS LEVELS AND THE CYTOKINE PROFILE IN PATIENTS WITH ALLERGIC DISEASES ASSOCIATED WITH CHRONIC EPSTEIN-BARR VIRAL INFECTION S. O. Zubchenko Danylo Halytsky Lviv National Medical University, Lviv, Ukraine Abstract. Advanced glycation end-products (AGEs) serve as densitometric markers of oxidative stress and inflammatory processes in many diseases and their complications. Objective: analysis of the correlation between AGEs and the cytokine profile in patients with allergic diseases associated with chronic Epstein-Barr viral infection (EBV-infection) in the active and latent phases. Object and methods. Determination and comparative analysis of AGEs levels and cytokines IL-17, IL-33, TNF-α in 66 patients aged (32.4 ± 7.5) years with allergic diseases associated with chronic EBV-infection in the active and latent phases and in EBV-seronegative patients with allergic diseases. Results. When determining AGEs by fluorescence spectroscopy in three groups of patients with allergic diseases, there was a reliably smaller number of AGEs than in healthy individuals. Presumably 12.3 % higher levels of IL-33 (p = 0.041) was determined in patients with active phase of EBV-infection compared to EBV-seronegative individuals, and in patients with latent phase of EBV-infection, the level of IL-17 was 3.54 times higher compared with EBVseronegative individuals (p = 0.011). It was determined that there is a reliable (p = 0.049) weak negative (r = -0.364) correlation between AGEs level and IL-33 in patients with allergic diseases associated with chronic EBV-infection in the active phase. It was investigated that no statistical difference between the levels of the proinflammatory cytokine TNF-α in the three groups was found. In patients with allergic diseases associated with chronic EBV-infection in the latent phase, a reliable (p = 0,032) weak negative (r = -0,364) correlation between AGEs and IL-17 was determined. Conclusions. Patients with allergic diseases compared to healthy individuals have lower levels of AGEs. AGEs play a significant pathogenetic role in the induction of allergic inflammatory process. AGEs accompanied by increased synthesis of IL-33 and IL-17 was more pronounced in patients with active phase EBV-infection, which was confirmed by clinical data. Key words: аdvanced glycation end-products, allergic diseases, chronic Epstein-Barr viral infection, cytokines. S. Zubchenko PhD, Associate Professor, Department of Clinical Immunology and Allergology Danylo Halytskyi Lviv National Medical University Pekarska street, 69, Lviv, Ukraine, 79010. Svitlanazu@gmail.com Аsthma and Allergy, 2021, 3, P. 30–35.