Open AccessMonitoring Methicillin-Resistant Staphylococcus Strains in the Moscow Medical and Surgical Center using Molecular-Biological Methods
Author(s) -
Т. С. Скачкова,
М. Н. Замятин,
О. А. Орлова,
Н. А. Юмцунова,
Н. Н. Лашенкова,
V.S. Fomina,
В.Г. Гусаров,
Andrey Shelenkov,
Ю. В. Михайлова,
Е. Н. Головешкина,
В. Г. Акимкин
Publication year - 2021
Publication title -
èpidemiologiâ i vakcinoprofilaktika
Language(s) - English
Resource type - Journals
eISSN - 2619-0494
pISSN - 2073-3046
DOI - 10.31631/2073-3046-2021-20-1-44-50
Subject(s) - medicine , staphylococcal infections , staphylococcus , microbiology and biotechnology , antibiotics , biology , staphylococcus aureus , bacteria , genetics
Relevance. Staphylococciareoneofthemostcommonpathogensintheetiologicalstructureofnosocomialinfections.MethicillinresistantstaphylococciareresistanttothemaingroupsofantibioticsandareoneofbacteriathatposethegreatestthreattohumanhealthaccordingtotheWorldHealthOrganization.Theintroductionofmodernmolecularbiologicalmethodsinthemonitoringo fm e thi c illin-r es i s tant staphylococci is necessary for the rapid and accurate identification of microorganisms, monitoring the epidemiological situation and studying the need for anti-epidemic measures. Aims. Аnalysis of the results of monitoring methicillinresistant staphylococcus strains using molecular biological methods in a multidisciplinary hospital in Moscow for one year.Materials and methods. Single-centerobservationalstudywitha one-year follow-up period (December 2016 to December 2017). The research included a molecular-biological analysis of biological material from 240 patients with signs of infection and washings samples (n=250) from the objects of the hospital environment. The whole genome sequencing was carried out for 24 samples, isolated from patients with different forms of staphylococcal infection and washings from a hospital environment. DNA detection of methicillin-resistant strains was performed using the «AmpliSens®MRSA-screen-titer-FL» reagent kit. Sequencing was performed on an Illumina HiSeq1500 instrument using the IlluminaHiSeq PE RapidClusterKit v2 and IlluminaHiSeqRapid SBS Kit v2.Results. DNAofmethicillin-resistantstaphylococciweredetectedin6.3%ofbloodsamplesfrompatientswithsignsofinfectionandin42.4%ofwashingsfromtheobjectsofthehospitalenvironment.TheresultsofPCRofwashingsamplesshowedthatDNAofmethicillin-resistantcoagulase-negativestaphylococciweredetectedmuchmoreoftenthanMRSA(p<0.001).DNAofmethicillin-resistantstaphylococciweredetectedmoreoftenintheintensivecareandintensivecareunitscomparedwiththehematologyandsurgicaldepartments(p<0.001).TheexaminesamplesofStaphylococcusaureusbelongedto6 different sequence types (ST-5, ST-7, ST-8, ST-22, ST-30 and ST-5555) and 8 spatypes (t008, t021, t091, t1062, t12437, t1544, t223, t4573). As a result of monitoring, an isolate with a new allelic profile was found. Today it has been assigned the number ST5555. The predominant type of staphylococcal mec cassette was the type IV SCCmec cassette.Conclusion. Duetothewidespreaddistributionofmethicillin-resistantstrainsandtheidentificationofepidemiologicallysignificantgeneticlinesofstaphylococci,itisnecessarytoconductregularmonitoring,takemeasurestolimitthespreadofsuchstrainsandintroducemodernmolecularbiologicalmethodsforquickandaccurateidentification.