Open AccessSTENOTROPHOMONAS MALTOPHILIA RESISTANCE AND BIODEGRADATION POTENTIAL
Author(s) -
Bella Babayan,
Marina A. Melkumyan,
Samvel A. Bagdasaryan
Publication year - 2020
Publication title -
chronos journal
Language(s) - English
Resource type - Journals
ISSN - 2658-7556
DOI - 10.31618/2658-7556-2020-29-2-2
Subject(s) - plasmid , stenotrophomonas maltophilia , microbiology and biotechnology , biology , bacteria , antibiotics , gene , transformation (genetics) , antibiotic resistance , pseudomonas , genetics , pseudomonas aeruginosa
In Current research the main goal was to study the resistance plasmids stability causes in S. maltophilia native strains. Using some microbiological, biochemical and genetical methods, it was noted, that the resistance to 13 mostly used in medicine, veterinary and agriculture antibiotics is stable and can be transmitted, even after the long-time cultivation without contact with any antibiotic. Then, using PCR, transformation analyses and xenobiotic tests, it was shown nucleoid and plasmid localization of studied genes. Polyphenol oxidases and caseinase genes were detected on nucleoid, while the lipases genes were indicated both on nucleoid and plasmids. It was found that biodegradation genes define the stabile replication of antibiotic resistance plasmids in a majority of S. maltophilia studied strains