Open AccessDIAGNOSIS OF BOVINE IMMUNODEFICIENCY VIRUS BY POLYMERASE CHAIN REACTION
Author(s) -
R.B. Akhmedov,
AUTHOR_ID,
I.Ya. Nam,
V.V. Zayakin,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2020
Publication title -
učenye zapiski kazanskoj gosudarstvennoj akademii veterinarnoj mediciny im. n.è. baumana
Language(s) - English
Resource type - Journals
ISSN - 2413-4201
DOI - 10.31588/2413-4201-1883-244-4-31-36
Subject(s) - virology , polymerase chain reaction , virus , biology , real time polymerase chain reaction , genetics , gene
The paper proposes and describes a method for PCR diagnostics of bovine immunodeficien-cy virus. The study included the selection of primers for PCR to detect BIV, optimization of con-centration and temperature-time conditions for PCR diagnostics of cattle immunodeficiency pro-virus, screening of cattle, and studying the features of BIV distribution. Based on the results of the presented study, primers for PCR diagnostics of BIV were selected and synthesized. Optimal con-ditions for PCR diagnostics of the virus were determined. 157 cattle were screened. 101 animals infected with VBI were identified. The wide prevalence of the virus in the territory of the Bryansk region was determined.