Open AccessMULTIPLEX PCR FOR INDICATION AND DIFFERENTIATION OF CABLES OF RESPIRATORY VIRAL INFECTIONS OF CATTLE
Author(s) -
Rostislav R. Larton,
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Konstantin A. Osyanin,
А. М. Алимов,
Tagir H. Faizov,
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Publication year - 2020
Publication title -
učenye zapiski kazanskoj gosudarstvennoj akademii veterinarnoj mediciny im. n.è. baumana
Language(s) - English
Resource type - Journals
ISSN - 2413-4201
DOI - 10.31588/2413-4201-1883-244-4-111-116
Subject(s) - virology , polymerase chain reaction , multiplex , multiplex polymerase chain reaction , biology , oligonucleotide , dna , microbiology and biotechnology , genetics , gene
Thus, as a result of the conducted polymerase chain reaction, it has been established that the mixture of oligonucleotide primers when interacting with one of the DNA markers does not inter-fere with accumulation of specific amplification products. Each biological pathogen corresponds an individual fluorescent marker for bovine viral diarrhea channel R6G, for parainfluenza-3-channel Fam, and for infectious rhinotracheitis Cy5.