Open AccessEvaluate the fresh and Post Thawing Quality of Holstein Friesian Bull semen
Author(s) -
Jamal Mehmood shah,
Tahir Hameed,
Farhat Abbas Bokhari,
Gul Zaman
Publication year - 2019
Publication title -
pak-euro journal of medical and life sciences
Language(s) - English
Resource type - Journals
eISSN - 2707-448X
pISSN - 2707-4471
DOI - 10.31580/pjmls.v1i1.961
Subject(s) - semen , sperm , semen quality , sperm motility , zoology , biology , ejaculation , andrology , semen collection , medicine , artificial insemination , endocrinology , pregnancy , genetics
TThe study was carried out during the yearr 2017 to determine the fresh and post-thaw quality of four Holstein Friesian bulls. Semen samples were examined for ejaculate volume, pH, mass activity, sperm concentration and sperm motility and sperm membrane integrity (HOST). The ejaculated semen volume of Holstein Friesian bulls was in the range of 6-8ml and differences in ejaculates were significant (P<0.01). The volume was significantly (P<0.05) higher (7.75ml) when collected on 5th July, slightly decrease in volume (7.25ml) when collected on 12th May and 26th July. Semen pH was higher (6.65) for 26th July ejaculation and lowest (6.55ml) for 24th May ejaculation. The results indicated that the semen of Holstein Friesian bulls did not have considerable variation in pH during May – July. Most of the semen samples were creamy white and yellow in colour, while few samples were milky white and watery. Mass activity score of semen samples indicated vigorous movement with moderate rapid waves and eddies. The sperm motility of fresh vs post-thaw semen was obtained 72.80 vs 48.75 percent (12th May), 75.00 vs 48.75 percent (24th May), 76.25 vs 55.00 percent (2nd June), 73.75 vs 52.50 percent (21st June), 75.00 vs 56.25 (5th July) and 75.00 vs 50.00 percent (26th July). In case of post-thaw semen, highest sperm motility of 56.25 percent was recorded in semen ejaculated on 5th July, while lowest post-thaw sperm motility of 48.75 percent was observed in semen ejaculated on 12th May and 24th May. Highest sperm concentration of 1549.75x106 was determined in semen ejaculated on 21.06.2017 and lowest concentration (1259.50x106) in 26.07.2017 collected samples. It was concluded that sperm motility was significantly (P<0.01) affected by semen types (fresh and post-thaw), while sperm concentration was also significantly (P<0.01) affected by ejaculation date and bulls. The membrane integrity (HOST) of the pooled data on fresh semen samples over a period of three months was 58.37 percent against post-thaw membrane integrity of 44.79 percent.