Open AccessIDENTIFYING OF DROUGHT TOLERANT WHEAT GENOTYPES IN CULTURE OF IMMATURE EMBRYOS IN VITRO
Author(s) -
Н.Н. Круглова,
O.A. Seldimirova,
А.Е. Zinatullina,
В. И. Никонов
Publication year - 2019
Publication title -
vestnik baškirskogo gosudarstvennogo agrarnogo universiteta
Language(s) - English
Resource type - Journals
ISSN - 1684-7628
DOI - 10.31563/1684-7628-2019-52-4-37-41
Subject(s) - biology , germination , seedling , agronomy , caryopsis , horticulture , phenology , drought tolerance , moisture , embryo , hybrid , botany , chemistry , organic chemistry , microbiology and biotechnology
The drought tolerance of immature embryos (17,5–20,0 days after artificial pollination) of a number of varieties and hybrid combinations of spring soft wheat was assessed. Donor plants were grown in the field during vegetation season 2018. The detection of drought resistance of immature embryos was conducted by their ability to give rise to a seedling developing to the phenophase of tillering in vitro in conditions simulating a moisture deficit. Moisture deficiency was achieved by introducing the PEG 6000 into composition of the nutrient medium MS. The embryos were incubated in the dark at +27 °C for 21 days. Conducted a visual assessment of morphological indicators of developing seedlings of regenerants to the phenological phase of tillering. The development of the seedling to the phenophase of complete grain ripeness in soil conditions ex vitro and the laboratory germination of the obtained grains were evaluated. The wheat genotypes, characterized by both the ability of immature embryos to form seedlings in the conditions of drought simulation and a sufficiently high laboratory germination of the obtained grains were revealed. These wheat genotypes can be recommended for use in the creation of drought-resistant hybrid lines and for their inclusion in the breeding process as initial forms.