Open AccessGranulomatosis as a component of proliferative reactions in the liver and pancreas after partial hepatectomy in the setting of superinvasive opisthorchiasis
Author(s) -
С. Д. Лазарев,
Р. М. Урузбаев,
Е. Д. Хадиева,
С. В. Куликова,
А. В. Косикина,
Л. В. Вихарева,
В. Г. Бычков
Publication year - 2021
Publication title -
journal of siberian medical sciences
Language(s) - English
Resource type - Journals
ISSN - 2542-1174
DOI - 10.31549/2542-1174-2021-2-65-76
Subject(s) - opisthorchiasis , pancreas , granuloma , pathology , opisthorchis , biology , medicine , liver fluke , gastroenterology , physiology , immunology , helminths
. Opisthorchiasis on the territory of Russia has a predominantly superinvasive form, i.e., the disease in hyperendemic foci proceeds with more severe manifestations and structural changes in the parasite’s ecological niche (liver, pancreas). With superinvasive opisthorchiasis (SO) in humans and animals, granulomatous infl ammation with the formation of granulomas is observed. Aimoftheresearch. To reveal the features of the granulomatous process in the liver and pancreas after partial hepatectomy in the setting of superinvasive opisthorchiasis in Syrian hamsters. Materials and methods. Research animals were divided into 2 groups: group 1 (n = 180) — SO modeling by introducing into the stomach of 50 Opisthorchis (O.) felineus metacercariae, superinvasions with 50 O. felineus metacercariae were repeated on the 6, 16th days after the primary infestation; group 2 (n = 86) — modeling of SO according to the same scheme as for group 1, and partial hepatectomy (PHE) (removal of the median lobe of an organ, 17.3–17.7% from its mass) on the 16th day after the last superinvasion. The animals were removed from the experiment on 7, 16th, 23rd, 32nd, 38, 120, 240th day. The study of liver and pancreas preparations was carried out using histological, histochemical, immunohistochemical methods. Results. The types, morphogenesis, course, outcomes of granulomas in the liver and pancreas were revealed. The factors of granuloma formation were the secretome, eggs and metabolites of O. felineus in both experimental groups. Conclusion. Granulomatosis in superinvasive opisthorchiasis, including after PHE, is an emergent state of the parasite-host system due to the accumulation of antigenic material in an amount exceeding the nonequilibrium state in this system. The regenerative process in the group of animals after PHE in the SO setting does not affect granulomatous infl ammation and the formation of granulomas.