Open AccessThe comparison of methods for the quantification of the pharmaceutical substance bismuth subsalicylate by UV-spectrophotometry and stripping voltammetry
Author(s) -
А.А. Vizer,
E. A. Ivanovskaya,
A.V. Ligostaev
Publication year - 2021
Publication title -
journal of siberian medical sciences
Language(s) - English
Resource type - Journals
ISSN - 2542-1174
DOI - 10.31549/2542-1174-2021-1-73-80
Subject(s) - spectrophotometry , detection limit , bismuth , chemistry , voltammetry , chromatography , correlation coefficient , linearity , analytical chemistry (journal) , stripping (fiber) , materials science , mathematics , electrochemistry , electrode , physics , statistics , organic chemistry , quantum mechanics , composite material
The results of a comparative study of methods for the quantitative determination of the active pharmaceutical substance (APS) bismuth subsalicylate by UV-spectrophotometry and stripping voltammetry (SV) are presented. Partial validation of the compared methods was carried out according to the following characteristics: linearity, detection limit, limit of quantification, accuracy. In the course of linearity assessment, the values of the correlation coefficient of the methods were established — 0.9987 for UV-spectrophotometry and 0.9982 for SV. The detection limit and the limit of quantification for UV-spectrophotometry were 2.35 and 7.12 μg/ml respectively, for SV — 1.16 and 3.52 μg respectively. The accuracy was determined by the constant term of the linearity equation, which was 3.34% of the interval’s middle value when analyzed by UV-spectrophotometry and 1.93% by SV. Thus, both methods can be used for the quantitative determination of the APS of bismuth subsalicylate; however, due to the fact that the content of bismuth is mainly determined in this case, the SV method is more preferable since it allows analyzing this particular part of the molecule.