Open AccessSynthesis and properties of oligochitosan ascorbate from Bombyx mori
Author(s) -
K.K. Pirniyazov,
В. Е. Тихонов,
С. Ш. Рашидова
Publication year - 2021
Publication title -
ķaraġandy universitetìnìn̦ habaršysy. himiâ seriâsy
Language(s) - English
Resource type - Journals
eISSN - 2663-4872
pISSN - 2518-718X
DOI - 10.31489/2021ch1/91-98
Subject(s) - bombyx mori , chitosan , depolymerization , chemistry , sodium nitrite , hydrolysis , molecular mass , ascorbic acid , food science , polymer chemistry , biochemistry , enzyme , gene
Oligochitosan samples were obtained by acid hydrolysis of high molecular weight chitosan isolated from Bombyx mori (B.M.). Carrying out acid hydrolysis for 6 hours, it was found that after 4 hours and further with increasing duration, the molecular weight of chitosan decreases to a value corresponding to the ranges of mo-lecular weights of oligochitosan 2–16 kDa. It has been seen that the optimal duration of hydrolysis, leading to the production of oligochitosan with a molecular weight of less than 16 kDa, should be considered 4–5 hours. Depolymerization of chitosan with a molecular weight of 177 kDa was carried out using sodium nitrite in so-lution to obtain oligochitosan with a molecular weight of 6 kDa. On the basis of oligochitosan samples ob-tained by two methods, their ascorbates were received. Under constant conditions with varying the ratio of the components (ChS:AA) and the pH of the solution, the reaction of chitosan ascorbate formation was car-ried out on the basis of the suspension method. The composition, structure, and molecular weight characteris-tics of oligochitosan ascorbate and oligochitosan Bombyx morisamples were confirmed by physicochemical methods. It has been seen that the obtained samples have antimicrobial properties against Fuzarium oxysporum.