Open AccessThe refinement of species affiliation of some click beetles (Coleoptera: Elateridae) based on the molecular genetic analysis
Author(s) -
T.M. Bragina,
D.T. Konysbayeva,
M.M. Rulyeva,
M.A. Bobrenko
Publication year - 2021
Publication title -
ķaraġandy universitetìnìṇ habaršysy. biologiâ, medicina, geografiâ seriâsy/k̦araġandy universitetìnìn̦ habaršysy. biologiâ, medicina, geografiâ seriâsy
Language(s) - English
Resource type - Journals
eISSN - 2663-5003
pISSN - 2518-7201
DOI - 10.31489/2021bmg1/14-19
Subject(s) - genbank , biology , fixation (population genetics) , dna barcoding , mitochondrial dna , dna sequencing , phylogenetic tree , genetic analysis , molecular evolution , evolutionary biology , zoology , gene , genetics
The article is devoted to data on the approbation of modern methods and the refinement of species affiliation of soil-inhabiting larvae of click beetles (wireworm) based on the molecular genetic analysis (DNAbarcoding). For the first time, data obtained on the complete identity of certain DNA sequences of a number of species of click beetles living in the Kostanay Region (Kazakhstan) — dangerous pests of agricultural crops. At the same time, the World genetic bank (GenBank) did not find identical DNA sequences of decoded DNA nucleotide sequences for a number of studied specimens. The basis for this study was the materials collected in 2018 in the subzone of ordinary black earth on sandy loam soils (Mendykarinsky district). The selection of larvae was carried out by the method of standard soil-zoological samples. The fixation and storage of the selected click beetles larvae was carried out according to the method of preparing samples for molecular genetic analysis with fixation in 96 % alcohol. After the classic identification of the taxonomic position of the collected specimens, the species were identified by genetic analysis on the nucleotide sequence of the cytochrome C oxidase I subunit gene (COI). The assembly and decoding of the DNA nucleotide sequences of the studied samples were carried out using the programs «Codon Code Aligner» and «MEGA-X». As a result of the work carried out in the DNA laboratory of the Museum of Natural History (University of Oslo, Norway), it was possible to identify the complete identity of the DNA sequences of several mass species of click beetles, while a number of decoded DNA sequences of model specimens were absent in the genetic bank, which requires replenishment in it with new data.