Open AccessDetection of phytopathogenic bacteria damaging weeping birch (Betula Pendula) by molecular identification method and сreation of phylogenetic genealogy
Author(s) -
Sardarbek Abiуev,
Aizhan Baubekova,
Р. З. Асилханова
Publication year - 2020
Publication title -
ķaraġandy universitetìnìṇ habaršysy. biologiâ, medicina, geografiâ seriâsy/k̦araġandy universitetìnìn̦ habaršysy. biologiâ, medicina, geografiâ seriâsy
Language(s) - English
Resource type - Journals
eISSN - 2663-5003
pISSN - 2518-7201
DOI - 10.31489/2020bmg4/8-15
Subject(s) - betula pendula , 16s ribosomal rna , biology , phylogenetic tree , botany , bacteria , gene , betula platyphylla , ribosomal rna , microbiology and biotechnology , genetics
The article discusses the morphological changes of birch trees (Betula pendula Roth.) due to bacterial cancer that grow in the artificial forest «Green Belt» surrounding the city of Nur — Sultan. Kernel samples were taken from diseased tree trunks, isolated bacterial cultures were grown in nutrient medium and given cultural and morphological characteristics. Extraction of cores from diseased birch trunks, isolation of pure strains of the pathogen, molecular identification of ribosomal RNA 16S nucleotide chains and comparison of these results with relevant data from the international database Gene Bank are carried out using modern methods in the field of bacteriology and botany. The molecular characteristics of the isolated bacterial strains corresponded to 96.72 % of the typical strain Dickeya dadantii based on the international Gene Bank.