Open AccessEffects of holothuria extract on pain behaviour and Fos like immunoreactivity (FLI) in formalin pain
Author(s) -
Che Badariah Aa,
Asma Ha,
Mohd Nizam H,
Siti Fa
Publication year - 2011
Publication title -
international medical journal malaysia/iium medical journal malaysia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.139
H-Index - 9
eISSN - 2735-2285
pISSN - 1823-4631
DOI - 10.31436/imjm.v10i1.701
Subject(s) - medicine , saline , analysis of variance , anesthesia , intraperitoneal injection , physiological saline , immunohistochemistry
The aim of this study was to determine the effects of gamat extract on pain behaviour and Fos like immunoreactivity (FLI) expression in the ventral posterolateral thalamus using the acute pain model. Materials & Methods: Fourteen Sprague-Dawley male rats (220-300 gram) were given intraplantar injection of 0.05ml formalin (1%) followed by intraperitoneal administration of either 4 mg/kg gamat extracts (Holothuria spp.) or saline (control). Behavioural changes were observed and rats were sacrificed 2 hours post-formalin injection. Immunohistochemistry testing was done on the brain sections. FLI was examined using a light microscope attached to an image analyser. The behaviour and FLI data were analysed using repeated measure analysis of variance and independent t-test respectively. Significance level was taken as p<0.05. Results: The control group has significantly higher pain scores compared to holothuria group (F (1) =13.635, p=0.003). There was significant reduction in the pain behaviour score in the holothuria group when compared to the control group in phase 1 (t (14) =2.9, p=0.012) and most of the time from 15 to 60 minutes post-formalin injection (t (12) =3.535, p=0.004). There was a significant reduction (P<0.05) in the number of FLI on the contralateral aspect of the ventral posterolateral thalamic nucleus in the group that received 4mg/kg of holothuria extract (63 3.18) compared to control group (84 6.36). Conclusion: This study showed that administration of holothuria extract significantly suppressed the pain behaviour and reduced the number of FLI in formalin injected rats compared to control.