Open AccessResults in the determination of some Hosta varieties by the method of isoelectric focusing
Author(s) -
A. Tilly Mándy,
Éva Stefanovits-Bányai,
Cs. Imre,
Z. Szafián
Publication year - 2001
Publication title -
international journal of horticultural science
Language(s) - English
Resource type - Journals
eISSN - 2676-931X
pISSN - 1585-0404
DOI - 10.31421/ijhs/7/1/256
Subject(s) - micropropagation , isozyme , phenology , biology , isoelectric focusing , peroxidase , botany , somaclonal variation , explant culture , enzyme , biochemistry , tissue culture , in vitro
Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.