Open AccessCallus induction on standard type Cymbidium cultivars
Author(s) -
L. Jánvári,
Gy. Bisztray,
T. Füzesi,
I. Velich
Publication year - 2000
Publication title -
international journal of horticultural science
Language(s) - English
Resource type - Journals
eISSN - 2676-931X
pISSN - 1585-0404
DOI - 10.31421/ijhs/6/4/236
Subject(s) - callus , explant culture , activated charcoal , tissue culture , thidiazuron , transformation (genetics) , somatic embryogenesis , botany , cultivar , biology , chemistry , horticulture , micropropagation , in vitro , biochemistry , organic chemistry , adsorption , gene
Tissue cultured Cymbidium PLBs (protocormlike body) were used as starting material to induce embryogenic callus which could serve as objects of genetic transformation. We obtained callus using two methods. The first method was culturing the PLB segments for one month in liquid MS medium in the presence of 0.5 mg/1 benzyladenine and 0.05 mg/1 naphtylacetic acid followed by cultivation on the same composition solid medium with 0.5 g/l activated charcoal for an additional month. Callus formation was observed on 30% of the explants. The second way was to propagate the PLB segments on solid MS medium supplemented with 1 mg/1 thidiazuron. In these cultures we also observed callus formation on 20% of the explants.