Open AccessFactors affecting rhizogenesis in vitro and acclimatisation of three cherry rootstocks
Author(s) -
Mohammed Hazim Askar Alsabbagh,
A. Abdul-Kader,
Mahmoud Khoder,
A. Kalhout
Publication year - 2000
Publication title -
international journal of horticultural science
Language(s) - English
Resource type - Journals
eISSN - 2676-931X
pISSN - 1585-0404
DOI - 10.31421/ijhs/6/1/64
Subject(s) - perlite , rootstock , auxin , acclimatization , horticulture , agar , botany , in vitro , peat , biology , ecology , biochemistry , genetics , bacteria , gene
Factors affecting rhizogenesis in vitro and acclimatisation of three rootstocks of cherry, i.e. Mahaleb, Maxma-14 and Weiroot -10 were investigated.
Rooting was easily achieved within 2-4 weeks on MS-based liquid or agar-gelled media containing auxins IBA at conc. 0.49 or 2.45 pM or NAA at conc. of 0.49 pM. On liquid media with 2.45 pM IBA, a maximum rooting efficiency of 95-100% was obtained. However, high concentrations of auxin delayed the time of root initiation for 3-5 days.
Rooted plantlets were transplanted into pots with a mixture of 3:1 (v/v) peat:perlite and acclimatised gradually to field conditions with efficiency of 60%.