Open AccessStudy of different factors of grapevine regeneration systems and genetic transformation
Author(s) -
Eduardo Irineu Novak,
A. Zok,
Róbert Oláh
Publication year - 2008
Publication title -
international journal of horticultural science
Language(s) - English
Resource type - Journals
eISSN - 2676-931X
pISSN - 1585-0404
DOI - 10.31421/ijhs/14/4/1529
Subject(s) - organogenesis , transformation (genetics) , regeneration (biology) , biology , agrobacterium , meristem , somatic embryogenesis , kanamycin , botany , stamen , shoot , microbiology and biotechnology , embryo , genetics , embryogenesis , gene , pollen
The most limitating factor for successful transformation is the absence of high-yielding regeneration protocols. However, the anther-derived embryogenic culture is an optimal technique for genetic transformation and it has been widely applied in many important cultivars, but the necessity of further development of regeneration systems has been proved. We attempted to produce somatic embryos on a wide range of genotypes from various tissues; leaves, petioles, stem segments. We started the examination of grapevine regeneration via organogenesis, succeeded in inducing shoot from the meristematic tissue of the base of bud by testing induction medium contained different concentrations of two types of hormones. To optimize the conditions of the Agrobacterium-mediated transformation, we studied the effectiveness of different Agrobacterium-treatments, the use of antioxidants and the sufficient quantity of kanamycin for selection of transformed cells.