Micropropagation of an orchid Dendrobium strongylanthum Rchb.f. | Zendy

Qiusheng Kong | Zendy; Shuai Yuan | Zendy; György Végvári | Zendy

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Micropropagation of an orchid Dendrobium strongylanthum Rchb.f.

Author(s) -

Qiusheng Kong,

Shuai Yuan,

György Végvári

Publication year - 2007

Publication title -

international journal of horticultural science

Language(s) - English

Resource type - Journals

eISSN - 2676-931X

pISSN - 1585-0404

DOI - 10.31421/ijhs/13/1/696

Subject(s) - explant culture , shoot , micropropagation , botany , dendrobium , murashige and skoog medium , sawdust , horticulture , biology , orchidaceae , chemistry , in vitro , ecology , biochemistry

A simple and reliable procedure for in vitro propagation of an orchid Dendrobium strongylanthum Rchb.f. was studied. Protochorm was induced from seed explants on 1/2 MS medium supplemented with 0.2 mg/L NAA. A mass of protochorm could be multiplied on proliferated medium of 1/2 Ms containing 0.5 mg/L V-6-BA. And bud differentiation of green global body was cultured in the same media, 2-2.5 cm shoots were formed after 30 day of culture. Addition of mashed banana and 0.5 mg/L NAA to 1/2 MS medium promoted root formation and vigorous growth. The plantlets were acclimatized and transplanted to compound materials of humus:sawdust (1:1) in greenhouse, the survival rate was more than 98%.

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