Open AccessResistance gene Sw-5 of tomato confers resistance to TCSV in Solanum melongena
Author(s) -
E. A. T. Picoli,
Gaus Silvestre Andrade Lima,
D. Lau,
Jéssica Fernandes Oliveira,
Marcelo Luiz de Laia,
Francisco Murilo Zerbini,
Sérgio Hermínio Brommonschenkel,
Miklós Fári,
Wagner Campos Otoni
Publication year - 2006
Publication title -
international journal of horticultural science
Language(s) - English
Resource type - Journals
eISSN - 2676-931X
pISSN - 1585-0404
DOI - 10.31421/ijhs/12/4/677
Subject(s) - biology , solanum , transgene , gene , southern blot , tospovirus , phenotype , melongena , horticulture , genetically modified crops , somatic embryogenesis , botany , genetics , virus , plant virus , tomato spotted wilt virus , embryogenesis
Eggplants transformed with Sw-5 gene, regenerated by organogenesis and somatic embryogenesis, were resistant to the Tomato chlorotic spot virus, while wild plants did present systemic infection. TO plants were selfed and the segregation analysis of T1 and T2 generation indicated the existence of one or more insertion sites. Southern blot analysis confirmed one or two independent insertions in T2 plants. Different lesions associated with the insertion number were observed in TI and T2 plants. T2 plants with two copies displayed faster hypersensitive reactions and characteristic necrotic lesions that contrasted with slower responses and necrotic ring lesions in plants with one copy. These results suggest that the Sw-5 confers resistance to tospovirus in transgenic eggplants and that the resistant phenotype depends on the number of transgene copies.