Open AccessAssociation between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients
Author(s) -
Hajar Karaali,
Jamilah Borjac
Publication year - 2019
Publication title -
genetics and applications
Language(s) - English
Resource type - Journals
eISSN - 2566-431X
pISSN - 2566-2937
DOI - 10.31383/ga.vol3iss3pp9-16
Subject(s) - acute kidney injury , creatinine , kidney , kidney disease , medicine , haplotype , single nucleotide polymorphism , population , gastroenterology , genotype , pathology , biology , genetics , gene , environmental health
Background: Acute kidney injury is a common condition associated with longer hospital stay and increased mortality. Kidney injury molecule-1 is one of the early and sensitive biomarkers for acute kidney injury diagnosis. Therefore we examined the relationship between kidney injury molecule-1 gene polymorphism and acute kidney injury in Lebanese hospitalized patients. Methods: Genomic DNA was isolated from blood samples collected from 50 patients and 40 controls. Kidney injury molecule-1 exon 4 was amplified by polymerase chain reaction. The amplified products were sequenced. Serum creatinine and urea levels were measured. Results: Three out of the five known single nucleotide polymorphisms showed significant association with susceptibility to the disease (P ≤ 0.05). Data analysis implied that carriers of the risk allele of these 3 single nucleotide polymorphisms were more predisposed to acute kidney injury. No association was found between the studied nucleotides variations and creatinine/urea levels. Haplotype analysis showed high association of the block CTA with acute kidney injury incidence and high creatinine and urea levels. Conclusions: Our results suggest that polymorphisms in exon 4 of kidney injury molecule-1 in the Lebanese population may be associated with acute kidney injury.