Open AccessSpectrophotometric Determination of Cefixime Following Simple Diazotization and Coupling with ?-Naphthol
Author(s) -
Samar Ahmed Darweesh
Publication year - 2017
Publication title -
al-maǧallaẗ al-’irāqiyyaẗ li-l-’ulūm al-ṣaydalāniyyaẗ/iraqi journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.122
H-Index - 1
eISSN - 2521-3512
pISSN - 1683-3597
DOI - 10.31351/vol26iss2pp1-6
Subject(s) - molar absorptivity , hydrochloric acid , cefixime , sodium nitrite , chemistry , absorption (acoustics) , detection limit , color reaction , nitrite , chromatography , spectrophotometry , nuclear chemistry , materials science , inorganic chemistry , organic chemistry , cephalosporin , reagent , optics , nitrate , biochemistry , physics , composite material , antibiotics
Cefixime (CFX) was treated with sodium nitrite and hydrochloric acid for diazotization reaction followed by coupling with ?-Naphthol in alkaline medium to form, a yellow colored azo dye compound which exhibits maximum absorption (?max) at 412 nm where the concentration of (CFX) was determined spectrophotometrically. The optimum reaction conditions and other analytical parameters were evaluated. Beer’s law was obeyed in the concentration range of (1-20) ?g.mL-1 with a molar absorptivity of 34870.5 L.mol-1.cm-1. The limit of detection was found to be 0.1090 ?g.mL-1 and the Sandell's sensitivity value was 0.0130 ?g.cm-2. The proposed method could be successfully applied to the determination of (CFX) in pharmaceutical formulations.