Open AccessStudy on culture conditionsfor extracellular alginate lyase production by Bacillus velezensis AlgSm1
Author(s) -
Thi Thuy Hang Cao,
Mai Nhu Hieu Vo,
Duc Thinh Pham,
Ngoc Linh Nguyen,
Dinh Thuat Nguyen,
Thi Thanh Van Tran
Publication year - 2021
Publication title -
tạp chí khoa học và công nghệ việt nam (b, online) (vietnam journal of science and technology - most)/tạp chí khoa học và công nghệ việt nam (điện tử)/tạp chí khoa học và công nghệ việt nam (b, print) (vietnam journal of science and technology - most)
Language(s) - English
Resource type - Journals
eISSN - 2615-9759
pISSN - 1859-4794
DOI - 10.31276/vjst.63(3).19-23
Subject(s) - fermentation , bacteria , food science , chemistry , carbon source , enzyme , yeast extract , yeast , extracellular polysaccharide , bacillus (shape) , biosynthesis , lyase , biochemistry , polysaccharide , microbiology and biotechnology , biology , genetics
Alginate lyase is an enzyme that degrades alginate to create bioactive oligoalginate for application in medicine, industry, agriculture... In this study, cultural conditions of marine bacteria, Bacillus velezensis AlgSm1 have been investigated for producing alginate lyase with high content and activity. The nutritional components such as carbon source, nitrogen source, initial pH value, and cultural time were investigated. The results showed that the cultural conditions of B. velezensis AlgSm1 to biosynthesize alginate lyase with high activity were 5 mg/ml alginate, 0.8 mg/ml yeast extract; pH 5.5 and 18-hours fermentation at 28-30°C.