Open AccessOptimisation of analytical conditions for simultaneous determination of phenol and chlorophenols by capillary electrophoresis with indirect laser-induced fluorescence detection
Author(s) -
Manh Huy Nguyen,
Hồng Anh Dương,
Hūng Viêt Pham
Publication year - 2021
Publication title -
tạp chí khoa học và công nghệ việt nam (b, online) (vietnam journal of science and technology - most)/tạp chí khoa học và công nghệ việt nam (điện tử)/tạp chí khoa học và công nghệ việt nam (b, print) (vietnam journal of science and technology - most)
Language(s) - English
Resource type - Journals
eISSN - 2615-9759
pISSN - 1859-4794
DOI - 10.31276/vjst.63(11db).51-55
Subject(s) - chromatography , repeatability , capillary electrophoresis , detection limit , chemistry , borax , laser induced fluorescence , phenol , reproducibility , fluorescence , electrolyte , electrophoresis , capillary action , analytical chemistry (journal) , materials science , electrode , raw material , physics , organic chemistry , quantum mechanics , composite material
This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.