Open AccessAntibacterial and Antioxidant Studies of Green Synthesized Silver Nanoparticles using Azadirachta indica (Neem) Leaf Extract
Author(s) -
Bishow Regmi,
Tirtha Raj Binadi,
Sarb Narayan Jha,
Rajib Kumar Chaudhary,
Bhoj Raj Poudel,
Surendra Kumar Gautam
Publication year - 2021
Publication title -
international journal of applied sciences and biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2091-2609
DOI - 10.3126/ijasbt.v9i3.39069
Subject(s) - selected area diffraction , nuclear chemistry , absorbance , silver nanoparticle , crystallite , analytical chemistry (journal) , antibacterial activity , dpph , nanoparticle , materials science , azadirachta , band gap , chemistry , spectroscopy , ultraviolet visible spectroscopy , nanochemistry , silver nitrate , transmission electron microscopy , nanotechnology , antioxidant , crystallography , organic chemistry , chromatography , botany , physics , bacteria , optoelectronics , quantum mechanics , biology , genetics
Silver nanoparticles (AgNPs) have been synthesized by green synthesis using Azadirachta indica leaf extract as both reducing and stabilizing agent. Synthesis of colloidal AgNPs was monitored by UV- visible spectroscopy. The UV- visible spectrum showed a peak at 455 nm corresponding to the plasmon absorbance of the silver nanoparticles. Crystallite structure of silver nanoparticles was studied using X-ray diffraction (XRD) analysis which revealed the face-centered cubic structure (FCC) with average particle size of 8.9 nm, calculated using Debye-Scherrer’s equation. Transmission electron microscopy (TEM) image revealed the agglomeration of small grain with particle size ranging from 2 to 14 nm. FCC crystalline nature was also evident from selected area electron diffraction (SAED) analysis. High purity of as-synthesized AgNPs was analyzed using energy dispersive X-ray (EDX) spectroscopy. Band gap energy was calculated to be 2.7 eV from UV- Visible spectra. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was stabilized by AgNPs which reveals its antioxidant efficacy. Well diffusion method showed 7 mm to 12 mm zone of inhibition (ZOI) against Gram-positive and Gram-negative bacteria, respectively confirming the antibacterial potential of AgNPs.Int. J. Appl. Sci. Biotechnol. Vol 9(3): 220-226.