Open AccessCitric Acid Production by Wild and UV – Treated Strains of Aspergillus niger on Two Different Mineral Salt Media
Author(s) -
Valentine E. Anyanwu,
P. O. Okerentugba
Publication year - 2013
Publication title -
international journal of applied sciences and biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2091-2609
DOI - 10.3126/ijasbt.v1i3.8358
Subject(s) - citric acid , sucrose , fructose , food science , aspergillus niger , chemistry , salt (chemistry) , strain (injury) , yield (engineering) , nuclear chemistry , biochemistry , biology , organic chemistry , materials science , anatomy , metallurgy
Microbial production of citric acid by a novel Aspergillus niger EE-12 and its UV – treated strain (UV-1) were carried out in shake flask cultures using mineral salt media containing sucrose or fructose as the carbon and energy sources. The highest citric acid concentration (36.1g/l) was obtained with the UV – treated strain UV-1 after 144 hours in medium containing sucrose and this was significantly higher (p<0.05) than the concentration produced by its parent strain EE-12. Citric acid production using medium containing sucrose (Sucrose salt medium) was significantly higher (p<0.05) than from medium containing fructose (Fructose salt medium) for both organisms. Product and growth yield coefficients (YCA/S, YCA/F, YCA/X, YX/S, YX/F) and volumetric rates (QF, QS, QCA, QX) were studied and compared for each of the test organisms based on the media (sucrose and fructose salt media) using students t-test analysis. This research indicated that the fungi strains are efficient for citric acid production using sucrose based medium