Open AccessIn Vitro Studies on Alpha-amylase Inhibitory Activities of Zanthoxylum armatum of Mid-Western Regions of Nepal
Author(s) -
Deepak Dahal,
Anup Subedee,
Kalpana Khanal,
Ram Chandra Basnyat
Publication year - 2021
Publication title -
amrit research journal
Language(s) - English
Resource type - Journals
ISSN - 2738-9553
DOI - 10.3126/arj.v2i01.39912
Subject(s) - alpha amylase , acarbose , alpha glucosidase , amylase , chemistry , traditional medicine , in vitro , enzyme , food science , biology , biochemistry , medicine
The alpha-amylase inhibitors are used as a drug to control hyperglycemia in type II diabetes mellitus. The plant extract is a natural source to minimize toxicity and other side effects. The alpha-amylase inhibition assay shows that the methanol extracts of fruits, seeds, leaves, and barks of Z. armatum exhibit 50% alpha-amylase inhibition activity at concentration 47.73 μg/mL, 50.25 μg/mL, 67.25 μg/mL, 152.6 μg/mL respectively, and hexane extracts of fruits, seeds, leaves and barks of Z. armatum exhibits 50% alpha-amylase inhibition activity at concentration 86.24 μg/mL, 84.02 μg/mL, 98.8 μg/mL, 138.81 μg/mL respectively, whereas the 50% alpha-amylase inhibition activity of Acarbose (standard) was found to be 33.66 μg/mL. Therefore, methanol and hexane extracts of fruits, seeds, and leaves show potential alpha-amylase inhibition activity and can be used as anti-diabetes.