Open AccessProduksi Enzim Selulase olehAspergillus niger pada Ampas Sagu
Author(s) -
Nora Idiawati,
Elliska Murni Harfinda,
Lucy Arianie
Publication year - 2015
Publication title -
jurnal natur indonesia/jurnal natur indonesia
Language(s) - English
Resource type - Journals
eISSN - 2503-0345
pISSN - 1410-9379
DOI - 10.31258/jnat.16.1.1-9
Subject(s) - cellulase , aspergillus niger , fermentation , cellulose , hydrolysis , chemistry , food science , glycoside , substrate (aquarium) , biochemistry , organic chemistry , biology , ecology
Production of cellulase by Aspergillus niger was carried out by growing the cultureson sago waste. Sago waste containscellulose that has not been used optimally. Cellulose is a polysaccharide consisting of glucose monomers linked by β-1,4-glycosides bonds. Glycoside bonds in cellulose can be enzymatically hydrolyzed into glucose with cellulase enzymes. Solid fermentation used to produce cellulase on sago waste as substrate was influenced by pH (3 to 6), moisture content(40% to 85%), and fermentation time (4 to 10 days). Products of the cellulase enzyme activity was measured by phenolsulfuricacid method. The results showed that the highest cellulase enzyme activity was 0.172 U/mL obtained at 85%moisture content, pH 5, and 8 days of fermentation time.