Open AccessIsolasi dan Karakterisasi Inulinase dari Aspergillus niger Gmn11.1 Galur Lokal
Author(s) -
Saryono Saryono
Publication year - 2012
Publication title -
jurnal natur indonesia/jurnal natur indonesia
Language(s) - English
Resource type - Journals
eISSN - 2503-0345
pISSN - 1410-9379
DOI - 10.31258/jnat.11.1.19-23
Subject(s) - inulinase , inulin , chemistry , fructooligosaccharide , aspergillus niger , fructose , chromatography , food science , sephadex , ammonium sulfate precipitation , size exclusion chromatography , biochemistry , enzyme
Inulin is a naturally potential polysaccharide used to produced fructose and fructooligosaccharide. Inulinaseknown also as ß-fructosidase can hydrolise inulin to fructose or fructooligosaccharide. Inulinase production fromAspergillus niger Gmn11.1 isolated from dahlia tubers is conducted using medium containing 1% inulin and 0,2%yeast extract. The crude enzyme (filtrate culture) is purified by means of ammonium sulphate salt precipitation,followed by Sephadex G25 gel filtration column chromatography and DEAE cellulose anion exchanger columnchromatography. The result indicated that the enzyme had optimum pH and temperature of 4,6 and 450C, respectivelywith incubation time of 15 hours. The Km and Vmaxs values obtained from this experiment are 20 mg/ml and 0,769mg/ml/hours, respectively. Whereas the relative molecular weight of inulinase was monitored by SDS PAGE is 63KDa.