Open AccessKarakterisasi Molekuler Bakteri Probiotik Ikan Kerapu Bebek Berbasis Teknik 16S Ribosomal DNA
Author(s) -
Feliatra Feliatra,
Wahyudi Andrito
Publication year - 2018
Publication title -
jurnal natur indonesia/jurnal natur indonesia
Language(s) - English
Resource type - Journals
eISSN - 2503-0345
pISSN - 1410-9379
DOI - 10.31258/jnat.10.1.13-17
Subject(s) - bacillus cereus , biology , bacteria , microbiology and biotechnology , 16s ribosomal rna , probiotic , bacillus (shape) , ribosomal rna , strain (injury) , dna , gene , biochemistry , genetics , anatomy
The aim of research was to find the molecular characteristics of probiotic bacteria in the digestive tract of grouper fish (Chromileptes altivelis) based on 16S ribosomal DNA technique. The bacteria were isolated from stomach and intestine of the fish and were analized in the Marine Microbiology Laboratory of fishery and marine Science Faculty of Riau University. The bacterial DNA were isolated by using a PCR (polymerase Chain Reaction) and was conducted in the biotechnology laboratory of Diponegoro University, Semarang. The purified DNA was sequenced in the BPPTTangerang. Result shown that 6 bacterial species may be potential as probiotic. There were Bacillus velesensis strain CR-11, Vibio alginolyticus A3G-2, Bacillus cereus site2S, uncultured bacterium clone BB3S16S-17, Bacillus subtillus strain CICC10066, and Bacillus flexus strain LF-3. these bacteria grow well at pH 2 and this indicated one of probiotic bacteria characteristics.