Open AccessIn vitro antibacterial activity of biologically synthesised silver nanoparticles using Terminalia avicenoides extracts against multidrug resistant Staphylococcus aureus strains
Author(s) -
M Bobai,
L Danjuma,
N.M Sani
Publication year - 2022
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2022.11203
Subject(s) - staphylococcus aureus , antimicrobial , multiple drug resistance , silver nanoparticle , antibacterial activity , phytochemical , chemistry , microbiology and biotechnology , minimum inhibitory concentration , antibiotics , traditional medicine , biology , bacteria , medicine , biochemistry , nanoparticle , materials science , genetics , nanotechnology
Antimicrobial resistance is currently one of the risen concerns to global healthcare in the 21st century. The search for new phytochemicals that could be developed as drugs for treatment of infectious diseases consequently increased with medicinal plants extracts derived nanoparticles receiving greater attention. This study was carried out to determine invitro antimicrobial activity of biologically synthesized silver nanoparticles using Terminalia avicenoides extracts against multidrug resistant Staphylococcus aureus strains isolated from wound infections. Isolation and characterization of Staphylococcus aureus was carried out using standard phenotypic and genotypic methods. Antimicrobial activity of selected antibiotics, Terminalia avicenoides extracts and biologically synthesized silver nanoparticles against multidrug resistant Staphylococcus aureus was carried out using standard procedures. The results of the susceptibility profile showed Staphylococcus aureus isolates resistant to 8.18% to 100% conventional antibiotics used, but 100% sensitive to imipenem. Phytochemical analysis of the extracts revealed the presence of tannins, alkaloids, flavonoids, cardiac glycoside, phenols, saponins and terpenoids. The antimicrobial activity of the biologically synthesized silver nanoparticles against multidrug resistant Staphylococcus aureus ranged from 28.25±1.90–30.65±2.21 mm and showed significant difference (p<0.05). Comparative analysis of Terminalia avicenoides extracts and their respective biologically synthesized silver nanoparticles activity showed significant difference (p<0.05) with antimicrobial activity of silver nanoparticles having larger zones of growth inhibition (29.60±2.83mm) compared to that of extracts (19.88±13.09mm). Remarkably, Terminalia avicenoides extracts derived silver nanoparticles exhibit higher inhibitory effects against the multidrug resistant Staphylococcus aureus strains, hence, can further be study and develop for wound infections therapy.