
Cytotoxic and antiproliferative potential of methanolic extract of Mallotus phillippensis in MCF-7 cell line
Author(s) -
Bimitha Benny,
A Sandesh Krishna,
Sujith Samraj,
Preethy John,
Uma Radhakrishnan
Publication year - 2022
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2022.11202
Subject(s) - acridine orange , mcf 7 , cytotoxicity , phytochemical , apoptosis , mtt assay , ethidium bromide , cytotoxic t cell , viability assay , neutral red , hela , traditional medicine , cell culture , chemistry , staining , biology , microbiology and biotechnology , biochemistry , cancer cell , in vitro , medicine , cancer , human breast , dna , genetics
Mallotus philippensis is a shrub used for anti-filarial anti-bacterial anti-inflammatory anthelmintic and immune-regulatory purposes for centuries in the current study methanolic extract of M. phillippensis leaves was examined in vitro for cytotoxicity and apoptotic potential in (MCF-7), breast cancer cell lines Various biochemical tests used to investigate the phytochemical contents of the plant extract. Qualitative phytochemical analysis of M. phillippensis leaves revealed the presence of phenolic compounds steroids flavonoids alkaloids diterpenes and tannins The (MTT), dye reduction test was used to assess the cytotoxicity of the methanol extract of M. phillippensis (MCF-7), cells were grown in (RPMI), medium supplemented with (10%), serum and (1%), antibiotic and antimycotic solution M. phillippensis leaf extract reduced cell viability in a dose-dependent manner, with a half maximal inhibitory concentration (IC50), of (190g/mL), Acridine orange Ethidium bromide and fluoroprobe benzimidazole-carbocyanine iodide 5, 5’, 6, 6’-tetrachloro-1, 1’, 3,3’-tetra ethyl (JC-1), staining were used to examine the influence of the extract on the development of apoptotic characteristics in (MCF-7), cells. The (AO/EB), staining revealed apoptotic cells with orange-red fluorescence in extract-treated cells which increased in a dosedependent manner whereas control cells showed green fluorescence. Control cells stained with (JC-1), dye fluoresced red-orange whereas cells treated with extract showed red-green fluorescence whose intensity changed in a dose-dependent manner The methanolic extract of M. phillippensis leaves showed invitro cytotoxic potential as well as induced apoptotic cell death in human breast cancer (MCF-7), cell lines and may be studied further to generate a potent anti- cancer drug.