Open AccessScreening for cytotoxic activity of Habenaria longicorniculata J graham tubers- an in-vitro study
Author(s) -
Satish Bn,
Mallya Suma,
Vishwanatha
Publication year - 2020
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2020.9513
Subject(s) - hela , cytotoxicity , in vitro , cytotoxic t cell , traditional medicine , cell culture , microbiology and biotechnology , biology , mtt assay , chemistry , biochemistry , medicine , genetics
About: Habenaria longicorniculata J. Graham are tuberous orchid, the tubers utilized by flok healers in cancer managemnet, as a rejuvenator. A study has been planned to evaluate In-vitro cytotoxicity of tuber extract against selected cell lines. Materials and Methods: H. longicorniculata J.Graham identified, uprooted during their flowering time. Tuber extract of this plant used for its In-vitro cytotoxicity against selected cell lines of Human Breast cancer (MCF 7), Human Liver carcinoma (HepG2), and Human cervix adenocarcinoma (HeLa) cells as per standard protocol. Results: Tuber Extract exhibited a CTC50 value of >1000 on MCF 7, HepG2 and HeLa cell lines. The results from the MTT assay indicate that 72hr extract incubation with the combined extracts is toxic to the cells and the level of damage is concentration dependent.