Open AccessAntioxidant and Hepatoprotective properties of Helianthus annuus seed extract against paracetamol-induced liver toxicity
Author(s) -
Abena Amponsaa Brobbey,
Yakubu Jibira,
Baba Fuseini,
Richard Nii Lante Lamptey,
Joseph Kwasi Adu
Publication year - 2020
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2020.9512
Subject(s) - chemistry , antioxidant , gallic acid , quercetin , dpph , flavonoid , traditional medicine , lipid peroxidation , alkaline phosphatase , biochemistry , hepatoprotection , liver function , food science , pharmacology , enzyme , biology , glutathione , medicine
This experiment aimed to evaluate the antioxidant and hepatoprotective effect of methanolic extract of Helianthus annuus (sunflower) seeds against paracetamol induced liver injury. Four groups of rats (n = 3) were used and administered orally once daily with H. annus methanolic extract (100, 300, and 500 mg/kg) for 7 days, followed by the induction of hepatotoxicity using acetaminophen. The blood and liver samples were subjected to liver function biochemical and lipid peroxidation assay. The extract was also subjected to in-vitro antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl radical scavenging assay. The total phenolic content and total flavonoid content were also evaluated. The extract was found to have a total flavonoids of 7.72 ± 2.3 mg/g quercetin equivalence and total phenolic content of 40.60244 ± 1.14 mg/g Gallic acid equivalence. H. annus methanolic extract exhibited a significant decrease (P < 0.0001) in Alanine aminotransferase, alkaline phosphatase, and aspartate aminotransferase enzyme levels. The extract exhibited antioxidant activity and contained high total phenol content. H. annus methanol extract was found to possess a potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content.