Open AccessThermoxidized palm oil diet (TPO)-induced haematological derangements in rats is ameliorated by Aloe vera and Garlic
Author(s) -
Obeten Christiana Egong,
Ani Elemi John,
Ime Akaninyene Ubong,
Kokelu Anthony Nduka,
Okon Udemeobong Edet
Publication year - 2018
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2018.7401
Subject(s) - aloe vera , garlic oil , medicine , garlic powder , allium sativum , traditional medicine , chemistry , food science , biology , botany , raw material , organic chemistry
Objective(s): Therapeutic effects of garlic/Aloe vera on TPO-induced derangements in some haematological parameters and cytoarchitecture of the bone marrow in rats were studied. Materials and Methods: 30 male wistar rats weighing 200-250g were grouped into five (n=6): Control, TPO (TPO diet), TPO+G (TPO/Garlic), TPO+A (TPO/Aloe) and TPO+G+A (TPO/Garlic/Aloe). 15g of thermoxidized palm oil was mixed with 85g of rat chow to prepare TPO. Aloe gel and garlic juice were orally administered at doses of 6ml/kg and 2ml/kg respectively following lethality studies. After 3months, rats were sacrificed and blood collected via cardiac puncture for analysis. Results: From the result, TPO/Aloe showed a significant increase in food/water intake compared to control. TPO, TPO/Garlic and TPO/Garlic/Aloe had a significantly reduced body weight when compared to control but significantly higher in TPO/Aloe compared to control and other three groups. RBC, PCV and Hb was significantly decreased in TPO compared to control but was significantly higher in TPO/Garlic, TPO/Aloe and TPO/Garlic/Aloe compared to TPO. Total White blood cell was significantly lower in TPO/Garlic, TPO/Garlic/Aloe when compared to TPO but significantly higher in TPO/Aloe when compared to TPO/Garlic and TPO/Garlic/Aloe. MCV, MCH and MCHC showed a significant increase in TPO compared to control but decreased in TPO/Garlic, TPO/Aloe, TPO/Garlic/Aloe when compared to TPO. Histology of the bone marrow showed normal morphology in Control, TPO/Garlic, TPO/Aloe and TPO/Garlic/Aloe while some histological derangements were seen in TPO. Conclusion: Garlic and Aloe vera showed therapeutic efficacy in ameliorating haematological derangements caused by TPO consumption