Open AccessTo study the Photodynamic antimicrobial activity of Henna extract and preparation of topical Gel formulation
Author(s) -
Sudheendra Kulkarni,
Vanita Kale,
Ketki Velankar
Publication year - 2018
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2018.7303
Subject(s) - lawsone , antimicrobial , chemistry , chromatography , antibacterial activity , shampoo , photodynamic therapy , maceration (sewage) , traditional medicine , organic chemistry , bacteria , medicine , materials science , biology , genetics , composite material
Henna, known to possess antibacterial & antifungal activities is reported to contain naphthoquinone derivatives which have potential as photodynamic agents. Current study comprises of evaluation of Photodynamic antimicrobial activity of alcoholic extract of henna, followed by preparation & evaluation of a topical gel formulation. The alcoholic extract prepared by maceration was analyzed for phytochemicals. Lawsone (2- Hydroxy-1,4 naphthoquinone) was isolated from Henna and was characterized by UV, IR & MASS to confirm its structure. The HPTLC fingerprinting of extract and quantification of lawsone in extract and fraction was carried out. The effect of light was tested on extract and fraction to assess its photosensitizing property. The antimicrobial activity of the extract was tested where one set was exposed to light and other kept in dark and zone of inhibition calculated for both sets