Open AccessEffect of ethanolic extract of Reissantia indica on the human breast cancer cell line (mcf-7)
Author(s) -
P Gayathri,
D. Sathish Sekar,
Katarikonda Sudhakar,
M. Sangeetha,
D. Chamundeeswari
Publication year - 2018
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2018.7103
Subject(s) - mcf 7 , celastraceae , traditional medicine , cancer cell lines , cancer cell , cell culture , human breast , cancer , west bengal , biology , botany , medicine , genetics , socioeconomics , sociology
Reissantia indica belongs to the family Celastraceae, is a native shrub to Maharashtra and Bengal, India. Its distinct plant parts are said to possess different activities, natives of Maharashtra and Bengal are using the plant parts for various purposes. In this experiment, the effect of ethanolic extract to destroy the cancer cells was studied. Study was carried on with human breast cancer cell line (MCF-7). Preparation of cell line was done using the reagents under standard protocol. Effect of plant extract on the cancer cells were recorded at two different timings (24 & 48 Hrs) respectively. Various doses of plant extract and standard were taken and introduced into cell line. Using 96 well plates the cells were seeded and studied for the viability. From the tabulated records, it clearly shows us the dose dependent response for the inhibition of cells. The maximum concentration of the plant extract 100µg/ml was found to be effective