Open AccessAntimicrobial screening of free and bound flavonoid from the bark of Terminalia arjuna
Author(s) -
Pankaj Jaiswal,
Padma Kumar
Publication year - 2016
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2015.4606
Subject(s) - flavonoid , terminalia arjuna , antimicrobial , bark (sound) , traditional medicine , antibiotics , chemistry , botany , biology , microbiology and biotechnology , medicine , biochemistry , terminalia , ecology , antioxidant
Continuous use of antibiotics results in the increased number of antibiotic resistant strains of microorganisms. Thus, a diverse arsenal of new antimicrobial agents is urgently needed to combat the diminishing efficacy of existing antibiotics. Almost all parts of plants (roots, stem, leaves, flowers, fruits and seeds) have been reported to have one or the other important bioactive compound but very little has been reported from bark of the plant except for few. Present study is confined to explore bark of Terminelia arjuna for some bioactive compounds. T. arjuna bark was collected, air dried and soxhlet extracted by using standard method for flavonoid extraction. Bound and free flavonoid extracts were then screened for antimicrobial activity using Disc Diffusion Assay. MIC, MBC and TA of each extract was also calculated. Both bound and free flavonoid showed activity against all the selected pathogens but the maximum inhibition zone was observed against Agrobacterium tumifacians (IZ= 19mm, AI=1.461±0.010) & Bacillus subtilis (IZ= 16mm, AI= 1.230±0.098) by the bound and free flavonoid extract of the plant respectively. Results obtained advocates the use of bark of the selected plant for pharmaceutical purpose to combat upcoming resistant pathogens.